Review





Similar Products

collagenase type ii  (Worthington Biochemical)
99
Worthington Biochemical collagenase type ii
Collagenase Type Ii, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+%28type/pmc12811436-116-41-44?v=Worthington+Biochemical
Average 99 stars, based on 1 article reviews
collagenase type ii - by Bioz Stars, 2026-08
99/100 stars
  Buy from Supplier
collagenase type i solution  (Thermo Fisher)
98
Thermo Fisher collagenase type i solution
Collagenase Type I Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+%28type/pm42353161-198-7-11?v=Thermo+Fisher
Average 98 stars, based on 1 article reviews
collagenase type i solution - by Bioz Stars, 2026-08
98/100 stars
  Buy from Supplier
collagenase iv  (Worthington Biochemical)
99
Worthington Biochemical collagenase iv
Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Collagenase Iv, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+%28type/pmc13052091-25-0-3?v=Worthington+Biochemical
Average 99 stars, based on 1 article reviews
collagenase iv - by Bioz Stars, 2026-08
99/100 stars
  Buy from Supplier
ls004189  (Worthington Biochemical)
99
Worthington Biochemical ls004189
Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Ls004189, supplied by Worthington Biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+%28type/pmc13052091-25-6-3?v=Worthington+Biochemical
Average 99 stars, based on 1 article reviews
ls004189 - by Bioz Stars, 2026-08
99/100 stars
  Buy from Supplier
ls004186  (worthington biochemical)
99
worthington biochemical ls004186
Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Ls004186, supplied by worthington biochemical, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+%28type/pmc13091043-37-0-4?v=worthington+biochemical
Average 99 stars, based on 1 article reviews
ls004186 - by Bioz Stars, 2026-08
99/100 stars
  Buy from Supplier
collagenase  (Thermo Fisher)
97
Thermo Fisher collagenase
Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Collagenase, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+%28type/pmc13214264-23-0-2?v=Thermo+Fisher
Average 97 stars, based on 1 article reviews
collagenase - by Bioz Stars, 2026-08
97/100 stars
  Buy from Supplier
collagenase type i  (Thermo Fisher)
97
Thermo Fisher collagenase type i
Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Collagenase Type I, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+%28type/pm42264325-201-19-22?v=Thermo+Fisher
Average 97 stars, based on 1 article reviews
collagenase type i - by Bioz Stars, 2026-08
97/100 stars
  Buy from Supplier
type i collagenase powder  (Thermo Fisher)
97
Thermo Fisher type i collagenase powder
Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following <t>collagenase-IV</t> treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.
Type I Collagenase Powder, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/collagenase+%28type/pm42251992-92-11-15?v=Thermo+Fisher
Average 97 stars, based on 1 article reviews
type i collagenase powder - by Bioz Stars, 2026-08
97/100 stars
  Buy from Supplier

Image Search Results


Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following collagenase-IV treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.

Journal: STAR Protocols

Article Title: Protocol for isolating viable human central nervous system T cells

doi: 10.1016/j.xpro.2026.104464

Figure Lengend Snippet: Temperature-induced gene artefacts Bulk RNA sequencing was performed on matched subcortical white matter (WM) CD11b + microglia and CD69 + CD8 + T cells from n = 4 brain donors. (A) Experimental overview. (B) Principal component analysis (PCA) of microglia and T cells using different dissociation methods. (C) Barcode plot showing enrichment of temperature induced signatures in microglia and T cells following collagenase-IV treatment at 37 0 C without transcription and translation inhibitors. (D) Gene expression of major brain T RM -cell markers after different dissociation procedures. (E) Expression of genes associated with T cells ( CD3D , CD8A ), B cells ( CD19 ), microglia ( P2RY12 ), and neurons ( MAP2 ) in the sequenced samples, obtained by RNA sequencing. Genes associated with astrocytes ( AQP4 ) and oligodendrocytes ( MAG ) were not detected. (F) Log-fold change of WM compared to circulating effector memory T (EM) cells (from Hsiao et al. ) to the heat-induced signature of T cells. Col-IV, collagenase IV; CPM, counts per million; FC, fold change; Inh., transcription and translation inhibitors; MG, microglia; T, T cell; T RM , tissue-resident memory T cell; WM, white matter.

Article Snippet: Collagenase IV , Worthington , Cat# LS004189.

Techniques: RNA Sequencing, Gene Expression, Expressing